| Product Name | Caspase 9 (p10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase 9 (p10)The activation of the initiator caspase, caspase 9, is regulated by the apoptosome, which is composed of cytochrome c bound to Apaf1 and dATP. The apoptosome responds to a diverse array of apoptotic stimuli that initiate the caspase cascade that ultimately results in cell death. The cascade of events starts with the proteolytic cleavage of caspase 9, that then cleaves downstream caspases, caspase 3, 6, and 7. The apoptotic initiating activity of caspase 9 can be regulated by XIAP, X-linked inhibitor-of-apoptosis protein; through the binding of caspase 9 at its amino terminus while it is bound to the apoptosome. This inhibition of caspase 9 activity has been shown to be relieved by Smac/DIABLO through its interaction with XIAP. |
| Immunogen | Synthetic peptide mapping to an internal domain of human Caspase 9/p10 subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/200. Predicted molecular weight: 46k precursor and 10k subunit. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602234 P55211 ICE-LAP6 842 CASPASE9c Caspase 9 p10 APAF3 Caspase9 p10 MCH6 CASP9 CASPASE-9c ICELAP6 APAF-3 APAF 3 ICE LAP6 CASPASE 9c |
|---|---|
| Target | Caspase 9 (p10) |
| Tissue Specificity | It could recognize mouse, rat, and human caspase-9 (p10) and caspase-9 precursor |
| Application Notes | WB: Use at a dilution of 1/200. Predicted molecular weight: 46k precursor and 10k subunit. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
