Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Caspase 9 antibody [N2C3] detects Caspase 9 protein at cytoplasm and nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Caspase 9 antibody [N2C3] diluted at 1:400.| Product Name | Caspase 9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Caspase-9 (caspase 9, apoptosis-related cysteine peptidase)This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspase 9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Dog, BovineDog (80%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APAF-3 antibody APAF3 antibody CASPASE-9c antibody ICE-LAP6 antibody MCH6 antibody CASP9 antibody apoptotic protease MCH-6 antibody caspase-9 antibody apoptotic protease activating factor 3 antibody "protein phosphatase 1 regulatory subunit 56 an |
|---|---|
| Target | Caspase-9 |
| Molecular Weight(MW) | 46 kDa (note) (2) |
Application
Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 9 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Caspase 9 antibody [N2C3] detects Caspase 9 protein at cytoplasm and nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Caspase 9 antibody [N2C3] diluted at 1:400.| Positive Control | HeLa (30 μM cisplatin treatment for 24 hr) , *NTUB1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
