Application
Human Lung (formalin-fixed, paraffin-embedded) stained with CASZ1 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.Application
Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD) Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot) Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown)| Product Name | CASZ1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CASZ1 |
| Immunogen | Synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein according to accession number CAI22571. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB (1:1000 - 1:3000), ELISA (1:25000 - 1:100000), IHC-P (5 µg/ml): |
|
| Species Reactivity | HumanMouseRatChimpanzeeBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CST FLJ12223 FLJ20321 SRG ZNF693 dJ734G22.1 |
|---|---|
| Tissue Specificity | human Cas5 protein. A BLAST analysis was used to suggest cross reactivity with Cas5 from mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross rea ... |
Application
Human Lung (formalin-fixed, paraffin-embedded) stained with CASZ1 antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Application
Western blot of affinity purified anti-hCas5 antibody shows detection of a band ~125 kD in size corresponding to hCas5 (arrowhead). Lane 1 contains a lysate from AS cells transfected with empty vector Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kD) Lane 3 contains lysate from BE2 cells that have been shown to contain a high level of hCas mRNA (as tested by Northern blot) Lane 4 contains lysate from human muscle tissue that also shows high levels of hCas mRNA Specific band reactivity is effectively competed when the antibody is pre-incubated with the immunizing peptide (data not shown)| Application Notes | WB (1:1000 - 1:3000), ELISA (1:25000 - 1:100000), IHC-P (5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
