Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4 antibody at 1:500 dilution.Application
CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: A375 7.5% SDS PAGE diluted at 1:5000Application
Sample (50 ug of whole cell lysate) A: mouse cerebellum 7.5% SDS PAGE diluted at 1:1000| Product Name | Cav beta 4 antibody , C-term |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cav beta 4This gene encodes a member of the beta subunit family of voltage-dependent calcium channel complex proteins. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein encoded by this locus plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CACNB4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Chicken, Rhesus Monkey, BovinePig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAB4 antibody CACNLB4 antibody EA5 antibody EIG9 antibody EJM antibody EJM4 antibody EJM6 antibody CACNB4 antibody "dihydropyridine-sensitive L-type calcium channel beta-4 subunit antibody" calcium channel voltage-dependent subunit beta 4 antibod |
|---|---|
| Target | Cav beta 4 |
| Molecular Weight(MW) | 58 kDa (note) (11) |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using CACNB4 antibody at 1:500 dilution.
Application
CACNB4 antibody [C3], C-term detects CACNB4 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CACNB4 protein stained by CACNB4 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: 293T B: HepG2 C: A375 7.5% SDS PAGE diluted at 1:5000
Application
Sample (50 ug of whole cell lysate) A: mouse cerebellum 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , HepG2 , A375 , mouse cerebellum |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
