Application
Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1 (Phospho-Tyr14)Application
Western blot analysis of extracts from 3T3 cells untreated (lane 1) or treated with H2O2 (lane 2) using Caveolin-1 (Phospho-Tyr14)| Product Name | Caveolin 1 (phospho Tyr14) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caveolin-1 (Phospho Tyr14)The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 14 (H-L-Y(p)-T-V) derived from Human CAVEOLIN-1. |
| Modification | p-Tyr14 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BSCL3 CGL3 MSTP085 VIP21 CAV1 cell growth-inhibiting protein 32 caveolin-1 caveolin 1 caveolae protein 22kDa |
|---|---|
| Target | Caveolin-1 (Phospho Tyr14) |
| Molecular Weight(MW) | 24 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1 (Phospho-Tyr14)
Application
Western blot analysis of extracts from 3T3 cells untreated (lane 1) or treated with H2O2 (lane 2) using Caveolin-1 (Phospho-Tyr14)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
