Application
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.Application
Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000Application
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2 antibody at 1:500 dilution.Application
Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments. IP samples: A549 whole cell extract A. 30 μg A549 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 μg Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | Caveolin 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caveolin 2 (caveolin 2)The protein encoded by this gene is a major component of the inner surface of caveolae, small invaginations of the plasma membrane, and is involved in essential cellular functions, including signal transduction, lipid metabolism, cellular growth control and apoptosis. This protein may function as a tumor suppressor. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Two transcript variants encoding distinct isoforms have been identified for this gene. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by one transcript. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Caveolin 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Cat, Pig, Rabbit, Sheep, Rhesus Monkey, Chimpanzee, BovineCat (100%), Pig (100%), Rabbit (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAV antibody MGC12294 antibody CAV2 antibody caveolin-2 antibody caveolin 2 isoform a and b antibody "caveolae protein 20-kD antibody" caveolin 2 antibody |
|---|---|
| Molecular Weight(MW) | 18 kDa (note) (2) |
| Cellular Localization | Golgi apparatus membrane; Single-pass membrane protein , Cell membrane; Single-pass membrane protein , Membrane , caveola; Single-pass membrane protein , Golgi apparatus membrane , Cell membrane , Membrane , caveola |
Application
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Application
Caveolin 2 antibody detects Caveolin 2 protein at membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Caveolin 2 protein stained by Caveolin 2 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000
Application
Caveolin 2 antibody detects Caveolin 2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using Caveolin 2 antibody at 1:500 dilution.
Application
Caveolin 2 antibody immunoprecipitates caveolin 2 protein in IP experiments. IP samples: A549 whole cell extract A. 30 μg A549 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 μg Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | A549 , C8D30 , Rat muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
