Application
Immunofluorescence staining of methanol-fixed Hela cells using c-Cbl (phospho-Tyr700) Antibody .Application
Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl(phospho-Tyr700) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Phospho-Tyr700) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CBL (phospho Tyr700) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Cbl (Phospho Tyr700)Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y(p)-M-T) derived from Human c-Cbl. |
| Modification | p-Tyr700 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C-CBL CBL2 RNF55 CBL casitas B-lineage lymphoma proto-oncogene E3 ubiquitin-protein ligase CBL signal transduction protein CBL proto-oncogene c-Cbl oncogene CBL2 RING finger protein 55 Cas-Br-M (murine) ecotropic retroviral transforming sequence |
|---|---|
| Target | c-Cbl (Phospho Tyr700) |
| Molecular Weight(MW) | 120 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using c-Cbl (phospho-Tyr700) Antibody .
Application
Western blot analysis of extracts from HT29 cells untreated or treated with UV using c-Cbl(phospho-Tyr700) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Cbl (Phospho-Tyr700) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
