Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CBP (Acetyl-Lys1535) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | CBP (acetyl Lys1535) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CBP (acetyl Lys1535)Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CBP around the acetylated site of Lys1535. |
| Modification | acetyl Lys1535 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-P |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CREBbinding protein CREB-binding protein CBP CREBBP EC=2.3.1.48 CREB binding protein CBP (acetyl K1535) CBP (acetyl Lys1535) CBP acetyl K1535 CBP acetyl Lys1535 Acetyl CBP (K1535) Acetyl CBP (Lys1535) |
|---|---|
| Target | CBP |
| Molecular Weight(MW) | 265 kDa (note) |
| Tissue Specificity | CBP (Acetyl-Lys1535) Antibody detects endogenous levels of total CBP protein only when acetylated at lysine1535. |
| Cellular Localization | Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. |
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using CBP (Acetyl-Lys1535) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
