Application
FACS ImageFlow Cytometry: CCR5 Antibody [] - Analysis of surface CCR5 expression on CEM.NKR.CCR5 cells. Cells were incubated with 25 ug/ml of rabbit anti-CCR5 2D7-2SK antibody and were detected with a FITC-labeled goat anti-rabbit IgG. Flow cytometry histogram shwoing staining of CEM.NKR cells (thin line) and CEM.NKR.CCR5 cells (thick line) or with isotype matched preimmune rabbit IgG control (dotted line). Image courtesy of Journal of Virology article (PMID: 15890918).Application
ICC/IF ImageImmunocytochemistry/Immunofluorescence: CCR5 Antibody [] - CCR5 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red)Application
WB ImageWestern Blot: CCR5 Antibody [] - WB Image of CCR5 in human liver lysate.| Product Name | CCR5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CCR 5 (C-C Chemokine Receptor 5)CCR5 is a major coreceptor for human HIV infection. |
| Immunogen | A synthetic peptide demonstrating a high affinity towards the monoclonal CCR5 2D7 (see reference 2). This peptide is known as the 2D7-2SK peptide and consists of the following residues: EWQKEGLVTLWLGGGGSC. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICC/IFWB |
| This antibody may be used for ELISA and flow cytometry, as well as being useful for inhibition of CCR5 tropic HIV envelope mediated fusion, syncytia formation, and infection in various in vitro assays. Suggested working dilutions: *ELISA: 1 ug/mlFlow cytometry: 25 ug/ml*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Gene Synonyms | CCR5 CCCKR5 CMKBR5 601373 CD195 CKR5 P51681 CC-CKR-5 CKR-5 1234 FLJ78003 IDDM22 CKR 5 CC CKR 5 |
|---|---|
| Target | CCR5 |
| Tissue Specificity | This antibody is specific for CCR5. It demonstrates 2D7-like reactivity. |
Application
FACS ImageFlow Cytometry: CCR5 Antibody [] - Analysis of surface CCR5 expression on CEM.NKR.CCR5 cells. Cells were incubated with 25 ug/ml of rabbit anti-CCR5 2D7-2SK antibody and were detected with a FITC-labeled goat anti-rabbit IgG. Flow cytometry histogram shwoing staining of CEM.NKR cells (thin line) and CEM.NKR.CCR5 cells (thick line) or with isotype matched preimmune rabbit IgG control (dotted line). Image courtesy of Journal of Virology article (PMID: 15890918).
Application
ICC/IF ImageImmunocytochemistry/Immunofluorescence: CCR5 Antibody [] - CCR5 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red)
Application
WB ImageWestern Blot: CCR5 Antibody [] - WB Image of CCR5 in human liver lysate.| Application Notes | This antibody may be used for ELISA and flow cytometry, as well as being useful for inhibition of CCR5 tropic HIV envelope mediated fusion, syncytia formation, and infection in various in vitro assays. Suggested working dilutions: *ELISA: 1 ug/mlFlow cytometry: 25 ug/ml*The investigator should determine the optimal working dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 48C. For long-term storage, aliquot into working volumes and store at -20 degrees Celcius. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml of purified rabbit sera. |
