Application
Western blot analysis of CD28 (pY218) expression in HeLa colchicine-treated (A); Jurkat (B); Raw264.7 colchicine-treated (C); H9C2 colchicine-treated (D) whole cell lysates.| Product Name | CD28 (phospho Tyr218) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD28 (phospho Tyr218)The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD28. The exact sequence is proprietary. |
| Modification | p-Tyr218 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD28 Tp44 CD28 molecule |
|---|---|
| Target | CD28 |
| Tissue Specificity | Recognizes endogenous levels of CD28 (pY218) protein. |
Application
Western blot analysis of CD28 (pY218) expression in HeLa colchicine-treated (A); Jurkat (B); Raw264.7 colchicine-treated (C); H9C2 colchicine-treated (D) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
