Application
CD3 gamma antibody [C1C3] detects CD3 gamma protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human PANC1 xenograft. CD3 gamma antibody [C1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:3000Application
CD3 gamma antibody [C1C3] detects CD3 gamma protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4ºC for 15 min. Black: Unlabelled sample was used as a control. Red: CD3 gamma antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.| Product Name | CD3 gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CD3G (CD3g molecule, gamma (CD3-TCR complex))The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD3 gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Pig, Rhesus MonkeyPig (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD3-GAMMA antibody FLJ17620 antibody FLJ17664 antibody FLJ79544 antibody FLJ94613 antibody MGC138597 antibody T3G antibody CD3G antibody "CD3g antigen gamma polypeptide (TiT3 complex) antibody" "CD3g molecule epsilon (CD3-TCR complex) antibody" |
|---|---|
| Target | CD3G |
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Membrane |
Application
CD3 gamma antibody [C1C3] detects CD3 gamma protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human PANC1 xenograft. CD3 gamma antibody [C1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:3000
Application
CD3 gamma antibody [C1C3] detects CD3 gamma protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4ºC for 15 min. Black: Unlabelled sample was used as a control. Red: CD3 gamma antibody [C1C3] dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.| Positive Control | Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
