Application
Western Blot analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | CD3 zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CD3 zeta(CD247) (CD3-zeta(CD247) molecule)The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD3 zeta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Sheep, BovineSheep (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CD3-ZETA antibody CD3H antibody CD3Q antibody CD3Z antibody T3Z antibody TCRZ antibody CD247 antibody "CD247 antigen zeta subunit antibody" TCR zeta chain antibody "CD3Z antigen zeta polypeptide (TiT3 complex) antibody" "T-cell antigen receptor |
|---|---|
| Target | CD3-zeta(CD247) |
| Molecular Weight(MW) | 19 kDa (note) (2) |
| Cellular Localization | Membrane |
Application
Western Blot analysis of CD247 expression in transfected 293T cell line by CD247 polyclonal antibody. A: Non-transfected lysate. B: CD247 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | CD3-zeta tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
