Rabbit Polyclonal antibody to CD44Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
Immunogen
Peptide sequence around aa.734~738(Q-N-V-D-M) derived from Human CD44.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ICC/IFWB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
CDW44 CSPG8 ECMR-III HCELL HUTCH-I IN LHR MC56 MDU2 MDU3 MGC10468 MIC4 Pgp1 CD44 heparan sulfate proteoglycan GP90 lymphocyte homing/adhesion receptor phagocytic glycoprotein 1 homing function and Indian blood group system cell surface gl
Target
CD44
Molecular Weight(MW)
80 (note)
Application
Application
Western blot analysis of extract from Hela cells using CD44 Antibody
Application
Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using CD44 Antibody
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.