Application
CD71 antibody detects CD71 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by CD71 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CD71 antibody , Internal |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD71 (transferrin receptor (p90, CD71))Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD71. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Dog, Cat, Pig, Rhesus Monkey, Bovine, Guinea pigDog (81%), Cat (86%), Pig (82%), Rhesus Monkey (94%), Bovine (84%), Guinea pig (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD71 antibody TFR antibody TFR1 antibody TRFR antibody TFRC antibody transferrin receptor protein 1 antibody "transferrin receptor (p90 CD71) antibody" |
|---|---|
| Molecular Weight(MW) | 85 kDa (note) (3) |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein , Melanosome , Transferrin receptor protein 1 , serum form: Secreted |
Application
CD71 antibody detects CD71 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD71 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CD71 antibody [N2C1], Internal detects CD71 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD71 protein stained by CD71 antibody [N2C1], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | Jurkat , Raji , NCI-H929 , K562 , HL-60 , NIH3T3 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
