Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using CD75 antibody at 1:500 dilution.Application
CD75 antibody [N2C3] detects CD75 protein at Golgi apparatus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. CD75 antibody [N2C3] diluted at 1:500.Application
Western Blot analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | CD75 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD75 (ST6 beta-galactosamide alpha-2,6-sialyltranferase 1)The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD75. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (87%), Rat (87%), Sheep (89%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC48859 antibody SIAT1 antibody ST6GalI antibody ST6N antibody ST6GAL1 antibody "CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 6-sialyltransferase 1 antibody" "CMP-N-acetylneuraminate beta-galactosamide alpha-2 6-sialyltransferase antibody" ST |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted |
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using CD75 antibody at 1:500 dilution.
Application
CD75 antibody [N2C3] detects CD75 protein at Golgi apparatus in human hepatocellular carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. CD75 antibody [N2C3] diluted at 1:500.
Application
Western Blot analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | CD75 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
