Application
Immunohistochemical staining of human tonsil stained with anti-CD79a antibodyApplication
Human leiomyoma stained with anti-CD79a antibody . Formalin-fixed tissues was boiled in 10 mM citrate buffer, pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes, then probed at a dilution of 1:100 for 10 minutes at RT.| Product Name | CD79a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CD79aThe CD79 molecule, comprising two polypeptide chains, mb-1 (CD79a) and B29 (CD79b), is physically associated in the B-cell membrane with immunoglobulins to constitute the B cell antigen receptor (BCR). The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. Almost all acute lymphoblastic leukemias of precursor B cell type are positive when tested with CD79a antibodies. |
| Immunogen | Synthetic peptide (Human and Mouse). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: Use at a concentration of 0.1 - 1.0 μg/ml. IHC-P: Use at a dilution of 1/100. WB: Use at a concentration of 0.5 - 2.0 μg/ml. Predicted molecular weight: 25 kDa. Sections require boiling in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MB-1 CD79A MB1 112205 973 IGA P11912 MB 1 |
|---|---|
| Target | CD79a |
| Cellular Localization | Cell Membrane |
Application
Immunohistochemical staining of human tonsil stained with anti-CD79a antibody
Application
Human leiomyoma stained with anti-CD79a antibody . Formalin-fixed tissues was boiled in 10 mM citrate buffer, pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes, then probed at a dilution of 1:100 for 10 minutes at RT.| Positive Control | Human tonsil sections |
|---|---|
| Application Notes | ELISA: Use at a concentration of 0.1 - 1.0 μg/ml. IHC-P: Use at a dilution of 1/100. WB: Use at a concentration of 0.5 - 2.0 μg/ml. Predicted molecular weight: 25 kDa. Sections require boiling in 10 mM citrate buffer, pH 6.0 for 10 min, followed by cooling at RT for 20 min Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS. pH 7.4 |
