Application
IHC analysis of human tonsil using CD8 antibody [EP1150Y] at a dilution of 1:250.Application
WB analysis of THP-1 lysate (10 μg) using CD8 antibody [EP1150Y] at a dilution of 1:1,000.| Product Name | CD8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1150Y] to CD8The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] |
| Immunogen | Synthetic peptide corresponding to residues near the C terminus of human CD8 alpha |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1150Y |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF：1/500. WB：1/1000. IHC-P：1/250 - 1/500. Optimal dilutions should be determined experimentally by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD8a molecule MAL Leu2 CD8A p32 |
|---|---|
| Target | CD8 |
Application
IHC analysis of human tonsil using CD8 antibody [EP1150Y] at a dilution of 1:250.
Application
WB analysis of THP-1 lysate (10 μg) using CD8 antibody [EP1150Y] at a dilution of 1:1,000.| Application Notes | ICC/IF：1/500. WB：1/1000. IHC-P：1/250 - 1/500. Optimal dilutions should be determined experimentally by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
