Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000| Product Name | CD86 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CD86 (B7-2 molecule)This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CD86. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B7-2 antibody B70 antibody CD28LG2 antibody LAB72 antibody MGC34413 antibody CD86 antibody "CD86 antigen (CD28 antigen ligand 2 B7-2 antigen) antibody" CTLA-4 counter-receptor B7.2 antibody FUN-1 antibody B-lymphocyte activation antigen B7-2 anti |
|---|---|
| Target | B7-2 |
| Molecular Weight(MW) | 38 kDa (note) (3) |
| Cellular Localization | Membrane |
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
