Application
Immunofluorescence analysis of methanol-fixed HeLa, using CD97 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using CD97 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | CD97 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CD97 (CD97 molecule)This gene is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules, likely encoded by a gene cluster on the short arm of chromosome 19. The encoded product is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. The protein has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. Alternative splicing has been observed for this gene and three variants have been found. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD97. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TM7LN1 antibody CD97 antibody seven-span transmembrane protein antibody seven transmembrane helix receptor antibody leukocyte antigen CD97 antibody CD97 antigen antibody "seven-transmembrane heterodimeric receptor associated with inflammation antibo |
|---|---|
| Molecular Weight(MW) | 92 kDa (note) (3) |
| Cellular Localization | Cell membrane , CD97 antigen subunit alpha: Secreted , extracellular space |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CD97 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded TOV21G xenograft, using CD97 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 B: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
