Application
IHC-P analysis of human breast carcinoma tissue using CDC2 (phospho Thr161) antibody.Application
WB analysis of extracts from COLO205 cells (Lane 1 and 2) and K562 cells (Lane 3) using CDC2 (phospho Thr161) antibody pre-incubated with or without blocking peptide.Application
IHC-P analysis of human breast carcinoma tissue using CDC2 (phospho Thr161) antibody pre-incubated with blocking peptide.| Product Name | CDC2 (phospho Thr161) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (phospho-Thr161)The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human CDK1 around the phosphorylation site of threonine161. |
| Modification | p-Thr161 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500-1:1000 IHC: 1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P34CDC2 CDK1 cyclin-dependent kinase 1 CDC28A |
|---|---|
| Target | CDC2 |
| Tissue Specificity | This antibody detects endogenous levels of CDC2 only when phosphorylated at threonine 161. |
Application
IHC-P analysis of human breast carcinoma tissue using CDC2 (phospho Thr161) antibody.
Application
WB analysis of extracts from COLO205 cells (Lane 1 and 2) and K562 cells (Lane 3) using CDC2 (phospho Thr161) antibody pre-incubated with or without blocking peptide.
Application
IHC-P analysis of human breast carcinoma tissue using CDC2 (phospho Thr161) antibody pre-incubated with blocking peptide.| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
