Application
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) AntibodyApplication
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Tyr15) Antibody .| Product Name | CDC2 (phospho Tyr15) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (Phospho Tyr15)Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y(p)-G-V) derived from Human CDC2. |
| Modification | p-Tyr15 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 CDC28A DKFZp686L20222 MGC111195 P34CDC2 CDK1 p34 protein kinase cell division protein kinase 1 cell cycle controller CDC2 cell division cycle 2 G1 to S and G2 to M cell division control protein 2 homolog cyclin-dependent kinase 1 |
|---|---|
| Target | CDC2 (Phospho Tyr15) |
| Molecular Weight(MW) | 34 (note) |
Application
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Tyr15) Antibody
Application
Western blot analysis of extracts from Hela cells untreated (lane 1) or treated with UV (lane 2) using CDC2 (Phospho-Tyr15) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: , ICC/IF: . Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
