Application
Western blot analysis of CDC2 (pY15) expression in HeLa UV-treated (A); A431 (B) whole cell lysates.| Product Name | CDC2 (phospho Tyr15) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (phospho Tyr15)The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDC2 (pY15). The exact sequence is proprietary. |
| Modification | p-Tyr15 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDK1 CDC28A P34CDC2 cyclin-dependent kinase 1 CDC2 |
|---|---|
| Target | CDC2 (phospho Tyr15) |
| Tissue Specificity | Recognizes endogenous levels of CDC2 (pY15) protein. |
Application
Western blot analysis of CDC2 (pY15) expression in HeLa UV-treated (A); A431 (B) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
