Application
Western blot analysis of extracts from Hela and MCF cells using CDC2 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed Hela cells using CDC2 Antibody .| Product Name | CDC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. |
| Immunogen | Peptide sequence around aa. 13~17 (G-T-Y-G-V) derived from Human CDC2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 CDC28A DKFZp686L20222 MGC111195 P34CDC2 CDK1 p34 protein kinase cell division protein kinase 1 cell cycle controller CDC2 cell division cycle 2 G1 to S and G2 to M cell division control protein 2 homolog cyclin-dependent kinase 1 |
|---|---|
| Target | CDC2 |
| Molecular Weight(MW) | 34 (note) |
Application
Western blot analysis of extracts from Hela and MCF cells using CDC2 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed Hela cells using CDC2 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
