Application
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min. Green: CDC2 protein stained by CDC2 antibody [N1], N-term diluted at 1:750. Blue: Hoechst 33342 staining.Application
Sample (30 µg of whole cell lysate) A: PC-12 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CDC2 antibody [N1], N-term diluted at 1:500.Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CDC2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC2 (cyclin-dependent kinase 1)The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CDC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovinePig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC2 antibody CDC28A antibody DKFZp686L20222 antibody MGC111195 antibody P34CDC2 antibody CDK1 antibody p34 protein kinase antibody cell division protein kinase 1 antibody cell cycle controller CDC2 antibody "cell division cycle 2 G1 to S and G2 |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in ice-cold MeOH for 5 min. Green: CDC2 protein stained by CDC2 antibody [N1], N-term diluted at 1:750. Blue: Hoechst 33342 staining.
Application
Sample (30 µg of whole cell lysate) A: PC-12 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CDC2 antibody [N1], N-term detects CDC2 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CDC2 antibody [N1], N-term diluted at 1:500.
Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 C: HCT116 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , H1299 , HCT116 , NIH-3T3 , JC , BCL-1 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
