Application
Western blot analysis of extracts from A549 cells treated with UV 15', using CDC25A (Phospho-Ser178) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | CDC25A (phospho Ser178) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CDC25A (phospho Ser178)Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC25A around the phosphorylation site of Ser178. |
| Modification | p-Ser178 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EC 3.1.3.48 CDC25M3 MPIP1 Dual specificity phosphatase Cdc25A EC3.1.3.48 Mphase inducer phosphatase1 CDC25A Mphase inducer phosphatase 1 M-phase inducer phosphatase 1 CDC25A2 M phase inducer phosphatase 1 CDC25A (phospho S178) CDC25A (phospho S |
|---|---|
| Target | CDC25A |
| Molecular Weight(MW) | 59 kDa (note) |
| Tissue Specificity | CDC25A (Phospho-Ser178) Antibody detects endogenous levels of CDC25A only when phosphorylated at serine178. |
Application
Western blot analysis of extracts from A549 cells treated with UV 15', using CDC25A (Phospho-Ser178) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25A (Phospho-Ser178) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
