Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A(Phospho-Ser76)Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF- alpha and CA using cdc25A (Phospho-Ser76) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A(Phospho-Ser76) Antibody (left) or the same antibody preincubated with blocking peptide(right)| Product Name | cdc25A (phospho Ser76) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cdc25A (Phospho Ser76)Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
| Immunogen | Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A. |
| Modification | p-Ser76 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC25A2 CDC25A cell division cycle 25A dual specificity phosphatase CDC25A CDC25A2-CAG isoform M-phase inducer phosphatase 1 cell division cycle 25 homolog A (S. pombe) |
|---|---|
| Target | cdc25A (Phospho Ser76) |
| Molecular Weight(MW) | 70 (note) |
Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A(Phospho-Ser76)
Application
Western blot analysis of extracts from Hela cells untreated or treated with TNF- alpha and CA using cdc25A (Phospho-Ser76) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A(Phospho-Ser76) Antibody (left) or the same antibody preincubated with blocking peptide(right)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
