(Rabbit polyclonal to exon 7 of human Cdc25a) and (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000,staining 500mg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)
Rabbit polyclonal to Cdc25ACdc25A is a 65kD protein phosphatase that plays an important role in cell cycle regulation. It appears to have a critical role in the regulation of G1/S transition of mammalian cells.
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Cell Division Cycle 25a encoded by exon 7 (LocusLink ID 993).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IPWB
For IP: Use at a concentration of 1 - 4 μg/ml. For WB: Use at a dilution of 0.1 - 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
993 116947 P30304 CDC25A2 CDC25A
Target
CDC25A
Application
Application
(Rabbit polyclonal to exon 7 of human Cdc25a) and (Rabbit polyclonal to exon 9 of human Cdc25a) both at a dilution of 1:2000,staining 500mg HeLa whole cell extract by Immunoprecipitation / Western Blot (ECL)
Application Notes
For IP: Use at a concentration of 1 - 4 μg/ml. For WB: Use at a dilution of 0.1 - 1.0 μg/ml. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
0.1ml Tris-citrate/phosphate, pH 7-8, with 0.1% sodium azide