Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C (Phospho-Ser216) Antibody .| Product Name | cdc25C (phospho Ser216) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cdc25C (Phospho Ser216)cdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. |
| Immunogen | Peptide sequence around phosphorylation site of serine 216 (S-P-S(p)-M-P) derived from Human cdc25C. |
| Modification | p-Ser216 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC25 CDC25C mitosis inducer CDC25 dual specificity phosphatase CDC25C M-phase inducer phosphatase 3 cell division cycle 25C protein phosphatase 1 regulatory subunit 60 phosphotyrosine phosphatase cell division cycle 25 homolog C (S. pombe) |
|---|---|
| Target | cdc25C (Phospho Ser216) |
| Molecular Weight(MW) | 60 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with serum using cdc25C (Phospho-Ser216) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C (Phospho-Ser216) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C (Phospho-Ser216) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
