Application
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C Antibody .Application
Western blot analysis of extracts from HT29 cells using cdc25C(Ab-216) Antibody and the same antibody preincubated with blocking peptideApplication
Western blot analysis of extracts from HT29 cells using cdc25C Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | cdc25C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cdc25Ccdc25C is highly conserved during evolution and it plays a key role in the regulation of cell division. The encodedprotein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. |
| Immunogen | Peptide sequence around aa.214~218 (S-P-S-M-P) derived from Human cdc25C. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDC25 CDC25C mitosis inducer CDC25 dual specificity phosphatase CDC25C M-phase inducer phosphatase 3 cell division cycle 25C protein phosphatase 1 regulatory subunit 60 phosphotyrosine phosphatase cell division cycle 25 homolog C (S. pombe) |
|---|---|
| Target | cdc25C |
| Molecular Weight(MW) | 60 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using cdc25C Antibody .
Application
Western blot analysis of extracts from HT29 cells using cdc25C(Ab-216) Antibody and the same antibody preincubated with blocking peptide
Application
Western blot analysis of extracts from HT29 cells using cdc25C Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25C Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
