Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDC25C antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDC25C antibody at 1:250 dilution.| Product Name | CDC25C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC25C (cell division cycle 25 homolog C (S. pombe))This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CDC25C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC25 antibody CDC25C antibody mitosis inducer CDC25 antibody dual specificity phosphatase CDC25C antibody M-phase inducer phosphatase 3 antibody cell division cycle 25C antibody "protein phosphatase 1 regulatory subunit 60 antibody" phosphotyrosin |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDC25C antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using CDC25C antibody at 1:250 dilution.| Positive Control | 293T , A431 , Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
