Application
Immunohistochemistry of APC3 in rat liver tissue with APC3 antibody at 5 μg/mL.Application
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CDC27 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CDC27The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | APC3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| WB: 1 - 2 ug/ml. IHC: 5 ug/ml: |
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| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | cell division cycle 27 D0S1430E D17S978E APC3 H-NUC CDC27Hs NUC2 ANAPC3 HNUC |
|---|---|
| Target | CDC27 |
Application
Immunohistochemistry of APC3 in rat liver tissue with APC3 antibody at 5 μg/mL.
Application
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC: 5 ug/ml: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Stored At 4ºc For Three Months And -20ºc, Stable For Up To One Year. As With All Antibodies Care Should Be Taken To Avoid Repeated Freeze Thaw Cycles. Antibodies Should Not Be Exposed To Prolonged High Temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
