Application
WB analysis of various cell lysates (50ug per lane) using CDC42 antibody at a dilution of 1:1000.Application
ICC/IF analysis of formaldehyde-fixed A431 cells using CDC42 antibody at a dilution of 1:20 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.Application
ICC/IF analysis of formaldehyde-fixed C6 cells using CDC42 antibody at a dilution of 1:20 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.Application
ICC/IF analysis of formaldehyde-fixed HepG2 cells using CDC42 antibody at a dilution of 1:200 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.Application
IHC-P analysis of human pancreas tissue with (left) or without (right) CDC42 antibody at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.| Product Name | CDC42 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CDC42The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013] |
| Immunogen | C-Terminal truncated human recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRatPrimate |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CDC42Hs G25K cell division cycle 42 |
|---|---|
| Target | CDC42 |
Application
WB analysis of various cell lysates (50ug per lane) using CDC42 antibody at a dilution of 1:1000.
Application
ICC/IF analysis of formaldehyde-fixed A431 cells using CDC42 antibody at a dilution of 1:20 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.
Application
ICC/IF analysis of formaldehyde-fixed C6 cells using CDC42 antibody at a dilution of 1:20 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.
Application
ICC/IF analysis of formaldehyde-fixed HepG2 cells using CDC42 antibody at a dilution of 1:200 (green), Phalloidin (red) and DAPI (blue). Images were taken at 60X magnification.
Application
IHC-P analysis of human pancreas tissue with (left) or without (right) CDC42 antibody at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol |
