Application
Western blot analysis of cdc42 in 3T3 (Lane 1) and Jurkat (Lane 2) cell lysate.| Product Name | cdc42/Rac antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to cdc42/Raccdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC42Hs G25K CDC42 |
|---|---|
| Target | cdc42/Rac |
| Tissue Specificity | This antibody is specific for cdc42/Rac. |
Application
Western blot analysis of cdc42 in 3T3 (Lane 1) and Jurkat (Lane 2) cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
