| Product Name | CDK8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Cdk8A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34 / Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression. The p54 / Cdk 8 interacts exclusively with Cyclin C. The Cdk 8 Cyclin C complex activates the kinase. Because of analogy with yeast Cdk 8, it has been proposed that the human Cdk 8 Cyclin C may interact with RNA polymerase, thus influencing the transcription mechanism(s) that lead to cell division. |
| Immunogen | Synthetic peptide: C-SQQPPQYSHQTHRY, corresponding to amino acids 451-464 of Human Cdk8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| Immunofluorescence: assay dependent. Immunoprecipitation (with protein A, native): use at a concentration of 10μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 54 kDa, which corresponds to the predicted molecular weight of Cdk 8.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | K35 1024 MGC126075 P49336 MGC126074 603184 CDK8 |
|---|---|
| Target | CDK8 |
| Cellular Localization | Nuclear and cytoplasmic. |
| Positive Control | HT29 cells |
|---|---|
| Application Notes | Immunofluorescence: assay dependent. Immunoprecipitation (with protein A, native): use at a concentration of 10μg / mg of lysate. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 54 kDa, which corresponds to the predicted molecular weight of Cdk 8.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
