Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE Centrin-1 antibody diluted at 1:1000Application
Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 μg of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Centrin 1 antibody detects Centrin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.| Product Name | Centrin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Centrin 1 (centrin, EF-hand protein, 1)The protein encoded by this gene plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. This encoded protein is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Centrin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (95%), Rat (95%), Xenopus laevis (84%), Chicken (84%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CEN1 antibody CETN antibody CETN1 antibody calcium binding protein antibody centrin-1 antibody caltractin antibody EF-hand protein antibody "centrin EF-hand protein 1 antibody" |
|---|---|
| Target | Centrin 1 |
| Molecular Weight(MW) | 20 kDa (note) (2) |
| Cellular Localization | Centrosome |
Application
Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE Centrin-1 antibody diluted at 1:1000
Application
Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 μg of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Centrin 1 antibody detects Centrin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody diluted at a dilution of 1:1000.| Positive Control | Jurkat , 293T , A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
