Application
Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1(Phospho-Ser280) AntibodyApplication
Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1 (Phospho-Ser280) Antibody .| Product Name | Chk1 (phospho Ser280) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Chk1 (Phospho Ser280)Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. |
| Immunogen | Peptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from Human Chk1. |
| Modification | p-Ser280 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CHK1 CHEK1 cell cycle checkpoint kinase Checkpoint S. pombe homolog of 1 serine/threonine-protein kinase Chk1 checkpoint kinase-1 CHK1 checkpoint homolog CHK1 checkpoint homolog (S. pombe) |
|---|---|
| Target | Chk1 (Phospho Ser280) |
| Molecular Weight(MW) | 56 (note) |
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1(Phospho-Ser280) Antibody
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1 (Phospho-Ser280) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
