Application
Western blot analysis of CHK1 (pS286) expression in HeLa (A); K562 (B); NIH3T3 (C); H9C2 (D) whole cell lysates.| Product Name | Chk1 (phospho Ser286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Chk1 (phospho Ser286)The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CHK1. The exact sequence is proprietary. |
| Modification | p-Ser286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CHK1 CHEK1 checkpoint kinase 1 |
|---|---|
| Target | Chk1 |
| Tissue Specificity | Recognizes endogenous levels of CHK1 (pS286) protein. |
Application
Western blot analysis of CHK1 (pS286) expression in HeLa (A); K562 (B); NIH3T3 (C); H9C2 (D) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
