Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser317) antibody diluted at 1:500.Application
Chk1 (phospho Ser317) antibody detects Chk1 (phospho Ser317) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk1 (phospho Ser317) protein stained by Chk1 (phospho Ser317) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Chk1 (phospho Ser317) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Chk1 (phospho Ser317) (checkpoint kinase 1)Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Chk1 (phospho Ser317). The exact sequence is proprietary. |
| Modification | p-Ser317 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Chk1 (phospho Ser317) phospho Chk1 (pS317) Chk1 phospho Ser317 phospho Ser317 Chk1 CHEK1 Chk1 (pSer317) Chk1 (pS317) checkpoint kinase 1 Chk1 (phospho S317) |
|---|---|
| Target | Chk1 (phospho Ser317) |
| Molecular Weight(MW) | 54 kDa (note) (6) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phospho Ser317) antibody diluted at 1:500.
Application
Chk1 (phospho Ser317) antibody detects Chk1 (phospho Ser317) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Chk1 (phospho Ser317) protein stained by Chk1 (phospho Ser317) antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HCT116 (30uM cisplatin treatment for 24h) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
