Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Citrate synthetase antibody [N2C3] dilution: 1:500.Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [N2C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Citrate synthetase antibody [N2C3] diluted at 1:500.Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [N2C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] . Western blot analysis was performed using Citrate synthetase antibody [N2C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of methanol-fixed A431, using Citrate synthetase antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Citrate synthetase antibody at 1:500 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Citrate synthetase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Citrate synthetase (citrate synthase)The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseChickenRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, BovineZebrafish (90%), Xenopus laevis (91%), Pig (95%), Bovine (95%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CS antibody, "citrate synthase, mitochondrial antibody", citrate synthase antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Mitochondrion matrix |
Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Citrate synthetase antibody [N2C3] dilution: 1:500.
Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody detects Citrate synthetase protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [N2C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Citrate synthetase antibody [N2C3] diluted at 1:500.
Application
Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [N2C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] . Western blot analysis was performed using Citrate synthetase antibody [N2C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of methanol-fixed A431, using Citrate synthetase antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Citrate synthetase antibody at 1:500 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
