Application
WB analysis of extracts from untreated or EGF-treated PC14 cells using c-Jun (phospho Ser73) antibody.Application
IHC-P analysis of human breast carcinoma tissue using c-Jun (phospho Ser73) antibody (left) or the same antibody pre-incubated with blocking peptide (right).| Product Name | c-Jun (phospho Ser73) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to c-Jun (phospho Ser73)This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human c- Jun around the phosphorylation site of serine 73. |
| Modification | p-Ser73 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500-1:1000 IHC: 1:50-1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1 JUN AP1 jun proto-oncogene |
|---|---|
| Target | c-Jun |
| Tissue Specificity | This antibody detects endogenous levels of c-Jun only when phosphorylated at serine 73. |
Application
WB analysis of extracts from untreated or EGF-treated PC14 cells using c-Jun (phospho Ser73) antibody.
Application
IHC-P analysis of human breast carcinoma tissue using c-Jun (phospho Ser73) antibody (left) or the same antibody pre-incubated with blocking peptide (right).| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
