Application
Immunofluorescence staining of methanol-fixed Hela cells using c-Jun (Phospho-Thr239) AntibodyApplication
Western blot analysis of extracts from Hela cells untreated or treated with UV using c-Jun(Phospho-Thr239) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Thr239) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | c-Jun (phospho Thr239) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Jun (Phospho Thr239)Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 239 (G-E-T(p)-P-P) derived from Human c-Jun. |
| Modification | p-Thr239 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AP-1, AP1, c-Jun, JUN, Jun activation domain binding protein, jun oncogene, v-jun avian sarcoma virus 17 oncogene homolog, p39, enhancer-binding protein AP1, proto-oncogene c-Jun, transcription factor AP-1, activator protein 1, v-jun sarcoma virus 17 onco |
|---|---|
| Target | c-Jun (Phospho Thr239) |
| Molecular Weight(MW) | 43 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using c-Jun (Phospho-Thr239) Antibody
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using c-Jun(Phospho-Thr239) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Thr239) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
