Application
Immunofluorescence analysis of methanol-fixed HeLa, using CKAP4 antibody at 1:200 dilution.Application
CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution.| Product Name | CKAP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CKAP4 (cytoskeleton-associated protein 4) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CKAP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLIMP-63 antibody, ERGIC-63 antibody, MGC99554 antibody, p63 antibody, CKAP4 antibody, 63 kDa membrane protein antibody, 63-kDa cytoskeleton-linking membrane protein antibody, "transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartm |
|---|---|
| Molecular Weight(MW) | 66 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum-Golgi intermediate compartment membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CKAP4 antibody at 1:200 dilution.
Application
CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 , MDA-MB-231 , *aortic smooth muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
