Application
Immunofluorescence analysis of methanol-fixed A549, using CKII alpha prime polypeptide antibody at 1:500 dilution.Application
CKII alpha antibody [N1C3] detects CKII alpha protein at cytoplasm in human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. CKII alpha antibody [N1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:3000| Product Name | CKII alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CKII alpha prime polypeptide (casein kinase 2, alpha prime polypeptide)Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CKII alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (98%), Rat (98%), Xenopus laevis (91%), Chicken (96%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CK2A2 antibody, CSNK2A1 antibody, FLJ43934 antibody, CSNK2A2 antibody, CK II alpha' antibody, casein kinase II subunit alpha' antibody, "casein kinase 2, alpha prime polypeptide antibody" |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed A549, using CKII alpha prime polypeptide antibody at 1:500 dilution.
Application
CKII alpha antibody [N1C3] detects CKII alpha protein at cytoplasm in human lung papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung papillary adenocarcinoma. CKII alpha antibody [N1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE diluted at 1:3000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
