Application
Western blot analysis of extracts from HL60 cells using c-kit Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | c-kit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-kitThis is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K) |
| Immunogen | Peptide sequence around aa.934~938 (H-I-Y-S-N) derived from Human c-Kit. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C-Kit CD117 PBT SCFR KIT mast/stem cell growth factor receptor Kit tyrosine-protein kinase Kit piebald trait protein proto-oncogene tyrosine-protein kinase Kit v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein soluble KIT variant |
|---|---|
| Target | c-kit |
| Molecular Weight(MW) | 145 (note) |
Application
Western blot analysis of extracts from HL60 cells using c-kit Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-kit Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
