Application
Immunofluorescence analysis of methanol-fixed HeLa, using CKMT2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using sMtCK antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Product Name | CKMT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CKMT2 (creatine kinase, mitochondrial 2 (sarcomeric))Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CKMT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Chicken, BovineMouse (91%), Rat (91%), Dog (94%), Pig (94%), Rabbit (92%), Chicken (84%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SMTCK antibody, CKMT2 antibody, sarcomeric mitochondrial creatine kinase antibody, S-MtCK antibody, basic-type mitochondrial creatine kinase antibody, "creatine kinase S-type, mitochondrial antibody", mib-CK antibody, "creatine kinase, mitochondrial 2 (sar |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using CKMT2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using sMtCK antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
