Application
WB analysis of Jurkat cells using Claudin 18 antibody at 0.2-1μg/ml. Lane A: marker Lane B: Jurkat cellsApplication
WB analysis of Jurkat cells using Claudin 18 antibody at 1μg/ml. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Product Name | Claudin 18 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Claudin 18This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010] |
| Immunogen | A synthetic peptide corresponding to a C-terminal region of Human Claudin 18 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 85%; Dog: 79%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SFTPJ CLDN18 SFTA5 |
|---|---|
| Target | Claudin 18 |
| Molecular Weight(MW) | 28 KDa (note) |
Application
WB analysis of Jurkat cells using Claudin 18 antibody at 0.2-1μg/ml. Lane A: marker Lane B: Jurkat cells
Application
WB analysis of Jurkat cells using Claudin 18 antibody at 1μg/ml. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Positive Control | Jurkat |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
