Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CSTF50 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cleavage stimulation factor antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE CSTF50 antibody diluted at 1:1000| Product Name | cleavage stimulation factor antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to cleavage stimulation factor (cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa)This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human cleavage stimulation factor. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chimpanzee, BovineMouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (98%), Pig (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CstF-50 antibody CstFp50 antibody CSTF1 antibody cleavage stimulation factor subunit 1 antibody cleavage stimulation factor 50 kDa subunit antibody CF-1 50 kDa subunit antibody CSTF 50 kDa subunit antibody "cleavage stimulation factor 3' pre-RNA s |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CSTF50 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using cleavage stimulation factor antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE CSTF50 antibody diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
