Application
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1500Application
CLPP antibody [C2C3], C-term detects CLPP protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: CLPP protein stained by CLPP antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
CLPP antibody detects CLPP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.| Product Name | CLPP antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CLPP (ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli))The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CLPP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLPP antibody endopeptidase Clp antibody "ClpP caseinolytic peptidase ATP-dependent proteolytic subunit antibody" "ClpP caseinolytic protease ATP-dependent proteolytic subunit homolog antibody" "putative ATP-dependent Clp protease proteolytic subuni |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using CLPP antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1500
Application
CLPP antibody [C2C3], C-term detects CLPP protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: CLPP protein stained by CLPP antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
CLPP antibody detects CLPP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CLPP antibody diluted by 1:500.| Positive Control | 293T , A431 , H1299 , HepG2 , Molt-4 , Raji , Jurkat , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
