Rabbit polyclonal antibody to c-Met (phospho Tyr1234)The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1234.
Modification
p-Tyr1234
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500-1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
AUTS9 met proto-oncogene (hepatocyte growth factor receptor) c-Met MET RCCP2 HGFR
Target
Met (phospho Tyr1234)
Tissue Specificity
This antibody detects endogenous levels of Met only when phosphorylated at tyrosine 1234.
Application
Application
WB analysis of extracts from untreated and HGF-treated A431 cells using Met (phospho Tyr1234) antibody.
Application Notes
WB: 1:500-1:1000:
Additional Information
Form
Liquid
Storage Instructions
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Storage Buffer
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.