Rabbit Polyclonal antibody to c-Met (phospho Tyr1235)The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human c-Met (pY1235). The exact sequence is proprietary.
Modification
p-Tyr1235
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
MET DFNB97 HGFR RCCP2 c-Met MET proto-oncogene receptor tyrosine kinase AUTS9
Target
c-Met
Tissue Specificity
Recognizes endogenous levels of c-Met (pY1235) protein.
Application
Application
Western blot analysis of c-Met (pY1235) expression in Hela (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.